insig1  (Santa Cruz Biotechnology)

Journal: The Journal of Infectious Diseases

Article Title: Characterization of the Spatiotemporal Localization of a Pan-Mucorales–Specific Antigen During Germination and Immunohistochemistry

doi: 10.1093/infdis/jiae375

Figure Lengend Snippet: Rhizopus arrhizus var delemar cells immunostained with mAb TG11 at different stages of spore swelling, germination, and hyphal growth. A , Composite photomicrographs (top) and individual channels (middle, bottom) of R arrhizus strain 99-880 with DAPI filters for CFW and Cy5 filters for TG11 + GAM-Cy5. Cells are stained with TG11 + GAM-Cy5 (bottom panel) and CFW (middle panel). Representative cells from liquid culture after 2, 3, 4, 5, 6, and 7 hours of incubation were imaged to visualize binding by mAb TG11 at different stages of cell growth. B , Time-lapse image series of a single live R arrhizus cell grown in media containing CFW and directly conjugated TG11-Cy5 and collected every 20 minutes. Note that indicated times are from the start of imaging with spores pregerminated for 2.5 hours. TG11 is seen to stain the growing hypha, highlighted in the adjacent schematic and Cy5 channel panels below. C , Representative negative controls: i , CFW, secondary only; ii , no CFW, TG11 + GAM-Cy5. Scale bars: 20 μm. CFW, calcofluor white; GAM-Cy5, Cy5-conjugated goat anti-mouse polyclonal IgG; mAb, monoclonal antibody.

Article Snippet: Rhizopus arrhizus var delemar , RA 99-880 , Reference strain , ATCC.

Techniques: Staining, Incubation, Binding Assay, Imaging

Journal: The Journal of Infectious Diseases

Article Title: Characterization of the Spatiotemporal Localization of a Pan-Mucorales–Specific Antigen During Germination and Immunohistochemistry

doi: 10.1093/infdis/jiae375

Figure Lengend Snippet: Different mucoralean fungi immunostained with mAb TG11. Composite photomicrographs taken with DAPI and Cy5 fluorescence filters: A – J , Rhizopus arrhizus var arrhizus MRL 21E307, Rhizopus microsporus FP469-12 , Mucor circinelloides MRL 22E56177 , Lichtheimia corymbifera MRL 20E42327 , Lichtheimia ramosa MRL 21E1059 , Cunninghamella bertholletiae CBS151.80 , Apophysomyces variabilis CBS658.93, Rhizomucor pusillus MRL 22E19013, Saksenaea erythrospora CBS138.279, and Syncephalastrum contaminatum MRL 22E22635. Cells are stained with TG11 + GAM-Cy5 (orange) and CFW (blue). CFW, calcofluor white; GAM-Cy5, Cy5-conjugated goat anti-mouse polyclonal IgG; mAb, monoclonal antibody. Scale bar indicates 10 μm.

Article Snippet: Rhizopus arrhizus var delemar , RA 99-880 , Reference strain , ATCC.

Techniques: Fluorescence, Staining

Journal: The Journal of Infectious Diseases

Article Title: Characterization of the Spatiotemporal Localization of a Pan-Mucorales–Specific Antigen During Germination and Immunohistochemistry

doi: 10.1093/infdis/jiae375

Figure Lengend Snippet: Transmission electron microscopy of immunogold-labeled Rhizopus arrhizus var delemar . Images of R arrhizus var delemar stained with ( A–C ) TG11 or ( D ) an IgG2b isotype control and a gold-conjugated secondary antibody. Gold particles are predominantly localized to ( B ) the hyphal cell wall as well as ( C ) the cytoplasm of germinating spores. B and C , Higher magnifications of the region identified in panel A by white squares. Note that panel C represents an independent slice of cells imaged in panel A .

Article Snippet: Rhizopus arrhizus var delemar , RA 99-880 , Reference strain , ATCC.

Techniques: Transmission Assay, Electron Microscopy, Labeling, Staining, Control

Journal: The Journal of Infectious Diseases

Article Title: Characterization of the Spatiotemporal Localization of a Pan-Mucorales–Specific Antigen During Germination and Immunohistochemistry

doi: 10.1093/infdis/jiae375

Figure Lengend Snippet: Ex vivo murine lung tissue infected with Rhizopus arrhizus var delemar and immunostained with mAb TG11. Photomicrographs of murine lung tissue uninfected (first row only) or infected with R arrhizus strain 99-880 or Aspergillus fumigatus strain A1160p + . Images were acquired with FITC (SYBR Green, column 2), DAPI (CFW, column 3), and Cy5 (mAb TG11-GAM; mAb JF5-GAM, column 4) fluorescence filters. Tissue slices are stained as follows: first, second, and fifth rows—mAb TG11 plus GAM-Cy5 (magenta); sixth and seventh rows—mAb JF5 plus GAM-Cy5 (magenta); third row—IgG2b isotype control plus GAM; fourth row—no primary plus GAM alone, CFW for fungal chitin (blue), and SYBR Green for DNA to reveal host cell nuclei (yellow). Note that lung connective tissue and alveolae also stain with CFW but not with mAb TG11-GAM or mAb JF5-GAM. Isotype control is a representative image for both conjugated antibodies. Images acquired in Z-stacks every 0.2 µm are presented as summed Z-projections. Scale bars: 10 μm. CFW, calcofluor white; GAM-Cy5, Cy5-conjugated goat anti-mouse polyclonal IgG; mAb, monoclonal antibody.

Article Snippet: Rhizopus arrhizus var delemar , RA 99-880 , Reference strain , ATCC.

Techniques: Ex Vivo, Infection, SYBR Green Assay, Fluorescence, Staining, Control